STK23 (SRPK3) (NM_014370) Human Recombinant Protein TP315496M from OriGene Technologies

Product Specs
ItemSTK23 (SRPK3) (NM_014370) Human Recombinant Protein
CompanyOriGene Technologies
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberTP315496M
TypeRecombinant Proteins
Molecular Weight61.8 kDa
Conjugate/TagC-Myc/DDK
Quantity100 µg
SpeciesHuman
NCBI Full Gene NameSRSF protein kinase 3
NCBI Gene AliasesMSSK-1, MSSK1, STK23
Molecule NameSRPK3
Source/Expression SystemHEK293T
Format25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Concentration>50 ug/mL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivitySRPK3 activity verified in a biochemical assay: SRPK3 (SRSF protein kinase 3) (TP315496) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. SRPK3 is a serine/threonine kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. Varying concentrations of SRPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors